Sea Buckthorn Juice Powder is rich in flavonoids. The extraction of flavonoids in the by-products of sea buckthorn can improve the income of enterprises and is of great significance to the development of sea buckthorn enterprises. Different extraction methods have different effects on the bioactive components of sea buckthorn. The different extraction and purification processes of sea buckthorn are compared below.
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Extraction Methods |
Advantages |
Disadvantages |
|
Organic solvent extraction |
Simple operation; Low requirements for Personnel and equipment |
Time consuming; High solvent consumption; There is a solvent residue problem. |
|
Supercritical CO2 Extraction |
Fast extraction; High safety factor; No reagent contamination |
High cost; Not suitable for industrial production; Instruments are expensive |
|
Microwave-assisted Extraction |
Short operating time; Less solvent consumption; High extraction efficiency |
Microwave radiation is harmful to human health. |
|
Ultrasonic Assisted Extraction |
Fast extraction; Efficient; Low solvent consumption; Low cost |
Destruction of active ingredients; Poor extract stability |
|
Subcritical Extraction |
Fast Extraction; Efficient; Fully retain active ingredients |
High cost; Less application |
The preliminarily extracted Sea Buckthorn Juice Powder flavonoids contain more non-flavonoid components such as inorganic salts, pigments and lipids. It needs further purification to get a stable product and use effect. The commonly used separation and purification methods are shown in the table below.
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Separation And Purification Methods |
Advantages |
Disadvantages |
|
Membrane Separation |
Low cost; high selectivity; wide application range; good separation effect; energy saving and environmental protection |
The filter membrane is easy to be polluted; Changes in permeability, irreversible. |
|
Thin Layer Chromatography |
Low cost; high throughput; low solvent consumption; ability to detect purity; fast separation; accurate results |
Long analysis time; complicated operation; poor repeatability |
|
Macroporous Resin Adsorption Method |
Wide application range; low cost; simple equipment; high product yield |
Poor purification rate; only suitable for water-soluble substances; susceptible to separation; poor adsorption and desorption capacity |
|
Recrystallization |
Easy to operate |
Difficult to remove water-soluble impurities |
|
high Speed Centrifugation |
The extract has high purity; the active ingredients are fully retained; It can be used for solid-liquid separation. |
Not suitable for small molecule compounds |
|
Metal Ion Complex Purification |
Strong specificity; simple process; high efficiency |
It is only suitable for a small number of compounds; The complex structure is difficult to remove. |