At present, Cordyceps Mushroom Extract Powder has achieved large-scale production, most of which are used in healthy food. In order to improve the efficacy and added value of the product, it is generally necessary to extract Cordyceps militaris before making related products. This paper introduces a extraction process for extracting active ingredients.
Due to the complex composition of Cordyceps sinensis, a series of impurities such as monosaccharides, water-soluble proteins and tannins are also raised during the extraction process. Difficulties in the purification of polysaccharides. Therefore, 85% ethanol was used in the experiment, and the Cordyceps militaris polysaccharide was removed by percolation method to remove impurities such as small molecular sugars and proteins, and then the extraction process of Cordyceps militaris polysaccharide was optimized. In the pilot test, 85% ethanol was also used to remove small molecular substances twice, and then the extraction process of Cordyceps polysaccharide was optimized.
The effects of extraction temperature, extraction time, solid-liquid ratio and extraction times on the extraction rate of Cordyceps polysaccharide and cordycepin were investigated by single-factor orthogonal experiment. We have established the best process for the extraction of Cordyceps polysaccharides and cordycepins. That is to say, the optimal conditions are: extraction temperature 100 °C, extraction time 2 h, solid-liquid ratio 1:10, extraction times 2 times, at this time, the extraction rate of cordyceps polysaccharide can reach 8.72%, and the extraction rate of cordycepin can reach 0.2908%.
This method is extracted by using a constant temperature mixer, and the experiment can be completed simply and effectively by setting the time and temperature with this instrument, and the extraction rate is higher than the traditional hot water extraction method and decoction method, which is a feasible and good method.
The extraction rate of crushed and uncrushed Cordyceps polysaccharides is not much different, so in actual production, it is not necessary to crush Cordyceps militaris skins to extract Cordyceps polysaccharides and cordycepins. The extraction of Cordyceps polysaccharide and cordycepin is directly carried out.