Soy isoflavones have various medicinal effects, so their extraction is of great significance. The main extraction methods of Soybean Extract Powder include organic solvent extraction, ultrasonic-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, subcritical water, solid phase extraction, enzymatic hydrolysis, and microbial fermentation.
8 Ways to Extract Soy Isoflavones
1. Organic solvent extraction method.
The organic solvent extraction method uses the principle of similar compatibility to extract soybean isoflavones from complex mixture components. The process of this method is simple, but this method will have residual organic solvent, and the recovery rate is low and the product purity is not high.
2. Ultrasonic-assisted extraction.
The ultrasonic-assisted extraction method uses the special effect of ultrasonic waves to accelerate the precipitation of soybean isoflavones from the mixture components. This method is simple in operation, low in cost, energy saving and fast in extraction, but the recovery rate is low.
3. Microwave-assisted extraction method
The microwave-assisted extraction method uses the electromagnetic field effect generated by the alternating current to make the molecules produce polarity exchange movement and speed up the formation of molecules.
Analysis of the. This method has high extraction efficiency, high product purity, short time, solvent saving, less pollution, but high cost, and is only suitable for trace and small amount of extraction and analysis.
4. Supercritical fluid extraction.
The supercritical fluid extraction method refers to that certain solvents can selectively change their solubility to different substances by changing the pressure and temperature in the supercritical state, so that the soybean isoflavones in the mixture can be separated out under different conditions. The solvent used is CO2. This method has high speed, high efficiency and high product purity, but the equipment is expensive and the operation is complicated.
5. Subcritical hydrolysis method.
The subcritical hydrolysis method means that the polarity of water can be changed by changing the temperature and pressure of water in a subcritical state (above 100°C but below the critical temperature of 374°C). Soy isoflavones can be extracted from the mixture under suitable conditions. This method is short in time, high in efficiency and less in pollution. It is a green technology, but the recovery rate is not high.
6. Solid phase extraction.
The solid-phase extraction method is to use the unique adsorption properties of certain solids to extract the extract from the liquid or solid phase. The commonly used solid phases for extracting soybean isoflavones are Strata-X and C18. This method is fast and reproducible, but the price of solid phase extraction column is high and the operation process is complicated.
7. Enzymatic hydrolysis.
The enzymatic hydrolysis method is to extract and catalyze soybean isoflavones from soybean by the catalysis of β-glucosidase. The content of free aglycone in the soybean isoflavones obtained by this method is greatly increased, but the cost is expensive and is rarely used in actual production.
8. Microbial fermentation.
The microbial fermentation method is to select suitable bacteria for fermentation in the medium with soybean as the nutrient substrate. After fermentation, elution and extraction are carried out to obtain soybean isoflavones. The method has high yield and is widely used, and can directly obtain free aglycones of soybean isoflavones, and can further improve the purity by cultivating excellent strains.
Summary
Soy isoflavones have great medical value as a pure natural active substance. For further research and development of soybean isoflavones, it is also necessary to find better extraction and purification methods for application in more fields. Qyherb Soybean Extract Powder is 40% active ingredient Isoflavones. Product quality assurance, fast delivery.