Magnolol and Honokiol are the main ingredients of Magnolia Officinalis Extract, which have good antibacterial and anticoagulant activities. The extraction methods of magnolol and magnolol from Magnolia officinalis mainly include solvent extraction, supercritical carbon dioxide extraction, enzymatic hydrolysis, high-pressure hot water extraction, etc., all of which have high extraction rates.
Magnolia Officinalis Extract Extraction Method
1. High pressure hot water extraction method
Studies have shown that the high-pressure hot water extraction method can obtain magnolol, honokiol and non-volatile components with high extraction rate in a short time, and the residue can be extruded into high-density plates.
2. Enzymatic extraction method
The enzymatic hydrolysis method was beneficial to improve the transfer rate of magnolol and magnolol in the water extract of Magnolia officinalis. The contents of honokiol and honokiol in the water extract of Magnolia officinalis after enzymatic hydrolysis treatment were determined by HPLC, and compared with the direct water extraction method.
After enzymatic hydrolysis of Magnolia officinalis, the transfer rates of honokiol and honokiol extracted by water reflux were 88.60% and 88.74%, respectively, which were higher than those of direct water extraction. The extraction efficiency obtained by compound enzyme pretreatment was higher than that obtained by single enzyme pretreatment.
3. Ultrasonic extraction method
The use of ultrasonic extraction of magnolol and honokiol can crush magnolia cells and release their contents during the extraction process, thereby shortening the extraction time, improving the extraction rate and reducing the production cost.
4. Supercritical carbon dioxide extraction method
The supercritical CO2 extraction of magnolol and honokiol has high extraction efficiency, good repeatability, stable and feasible process, and the extract has good antioxidant activity.
5. Solvent extraction method
The research shows that the optimal solvent extraction process of Magnolia officinalis by orthogonal design method is simple, reasonable, stable and feasible. The content of magnolol and honokiol in different extracts was determined by HPLC method at 70%, and the extraction effect of ethanol heat reflux was the best. Different extraction solvents had effects on the contents of honokiol and honokiol in Magnolia officinalis.
Summary
According to the different properties of honokiol and honokiol, various extraction and purification methods were adopted. Although this achieves a better extraction rate and purification rate, there are still some deficiencies. In the future, more in-depth research will be conducted on the extraction of magnolol and Magnolia officinalis, and an economical and feasible extraction and purification process will be found, which will lay a data foundation for the industrialization and standardization of Magnolia officinalis extraction.